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KMID : 0360220090500091404
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Journal of the Korean Ophthalmological Society
2009 Volume.50 No. 9 p.1404 ~ p.1408
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Effect of Bevacizumab on Survival and Production of Nitric Oxide in Trabecular Meshwork Cells
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Kim Sin-Hoo
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effect of bevacizumab, a monoclonal antibody against vascular endothelial growth factor (VEGF), on the survival and production of nitric oxide (NO) in human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to 0, 0.25, 1.0, and 2.5 mg/ml bevacizumab for 24 hours. Cellular survival and production of NO were assessed by MTT assay and Griess assay, respectively.
Results: Bevacizumab did not affect the cellular survival at low concentrations but decreased cellular survival significantly at high concentrations (>1.0 mg/ml) accompanied with increased NO production.
Conclusions: High concentrations of bevacizumab may be toxic to HTMC.
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KEYWORD
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Bevacizumab, Nitric oxide, Trabecular meshwork cells
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